Embryo transfer tips
and Answers about Embryo Transfer Products
What is the difference between the F17
21” deep chamber gun and the F17A 21”
A. The only
difference is the location of the internal backstop that the
cotton-plug end of the straw rests against. When inserted
into the gun,
approximately 2-3mm of straw must extend beyond the open end of the
order for the open end of the straw to engage and seal properly into
tip of the blue sheath. The deep chamber rod is designed for the full
(non-shortened) 132mm x 0.25cc straw, like what is used for fresh
straws sealed with a plastic plug. Straws that are sealed
freezing with a heat sealer or with PVC powder must then be clipped /
post thaw and require the F17A shallow chamber rod.
What is the difference between BioLife
A. Each is a
proprietary line of embryo collection / freezing media. BioLife is
made specifically for Agtech and Vigro products are made for
Each line has been sold commercially for many years.
I didn’t use all of the holding media, or ethylene glycol
contained in a bag, Can
I store it for later use?
you entered the bag each time with a new sterile needle, then the
contents can be refrigerated (not frozen) and used again for up to a
week. If the solution is clear and has been refrigerated,
most likely it
is fine to use. Always check for precipitate or particles coming out of
suspension. If in doubt, toss it out!
I am having a problem with embryo straws exploding upon thaw. The
or plastic plug blows out as soon as the straw hits the warm water
What could be causing
A. This is due to nitrogen seeping into the straw around or through the
during freezing. The nitrogen then expands rapidly during
either the plastic or cotton plug to blow, much like a bullet
out of a rifle.
There can potentially be several causes of this problem:
much fluid has been pulled through the plug, and the outer cotton plug
extremely saturated with fluid during freezing.
enough fluid has been pulled through the plug and the PVC is not
properly wetted or seated.
have been improperly stored in a humid environment causing degradation
partial wetting of the PVC portion of the plug prior to actual straw
can I do to stop my straws from exploding?
and refine your plug wetting technique.
Make sure you are completely wetting the PVC portion of
the plug and
stopping immediately after the entire PVC portion is noticeably wetted. Be sure you’re
not pulling too much fluid
through and saturating the outside cotton plug as well.
Freeze these straws as you normally would and
store them in nitrogen for a week or two.
Make detailed notes and identify what your technique is on
batch. Thaw the
straws and record your
store your straws in a dark, dry place.
Preferably in a zip-lock bag, or heat sealed bag.
such as Agtech item# F03
DT6300 are also believed to be less prone to
exploding by some ET practitioners.
experience has shown that all straws will function properly if the
sealing technique and straw storage is consistently exercised.
Embryo Transfer Tips and
you’re not ready to upgrade all the way to
aTLS-2200 labeling system, you can start by just using the PTL-19-427
labels. The labels lay flat and make a great surface for writing on
with a Sharpie marker. Then simply peel off the
wrap it around your straw. To some, this is much easier than
trying to write on
the straw or plug sidewall!
avoid spilling the contents of your flush
filter, be certain the outflow clamp below the EmCon
filter or Zona filter
open before allowing medium to flow toward the
Otherwise, pressure will build inside the filter and pop the lid off.
flushing large, deep-bodied cattle, it is
helpful to elevate the front of the cow by 8-10 inches. Build an
her front feet walk up onto. If flushing in a tie-stall barn
with a gutter,
position the donor so that her back feet stand in the gutter.
your search yields significantly less embryos
than what you expected from ovarian palpation, get the donor back in
reflush. It’s a win-win tactic. If the
reflush yields embryos this
indicates that the original flush procedure was inadequate.
additional embryos are collected from the second flush, this most
indicates that the ovulated oocytes/embryos were retained in the
are not accessible by our nonsurgical flush technique.